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epithelial growth kit  (ATCC)


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    ATCC epithelial growth kit
    Epithelial Growth Kit, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/epithelial growth kit/product/ATCC
    Average 94 stars, based on 19 article reviews
    epithelial growth kit - by Bioz Stars, 2026-03
    94/100 stars

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    C. trachomatis infection of primary cervical <t>epithelial</t> cells. A Growth curve for C. trachomatis infection in primary cervical epithelial cells over a time course of 96 h. The chlamydial genome copy number was normalized to that of the host and the results were expressed as mean ± standard error (SE) of two independent experiments performed in triplicates. Significance is indicated as * P < 0.05 and ** P < 0.01. B Representative images of C. trachomatis -infected primary cervical epithelial cells at 24 h.p.i., and 48 h.p.i. EBs (dashed arrows) and RBs (solid arrows) were stained green whereas cytoplasm was counterstained red. Scale bar: 100 μm
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    C. trachomatis infection of primary cervical <t>epithelial</t> cells. A Growth curve for C. trachomatis infection in primary cervical epithelial cells over a time course of 96 h. The chlamydial genome copy number was normalized to that of the host and the results were expressed as mean ± standard error (SE) of two independent experiments performed in triplicates. Significance is indicated as * P < 0.05 and ** P < 0.01. B Representative images of C. trachomatis -infected primary cervical epithelial cells at 24 h.p.i., and 48 h.p.i. EBs (dashed arrows) and RBs (solid arrows) were stained green whereas cytoplasm was counterstained red. Scale bar: 100 μm
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    C. trachomatis infection of primary cervical epithelial cells. A Growth curve for C. trachomatis infection in primary cervical epithelial cells over a time course of 96 h. The chlamydial genome copy number was normalized to that of the host and the results were expressed as mean ± standard error (SE) of two independent experiments performed in triplicates. Significance is indicated as * P < 0.05 and ** P < 0.01. B Representative images of C. trachomatis -infected primary cervical epithelial cells at 24 h.p.i., and 48 h.p.i. EBs (dashed arrows) and RBs (solid arrows) were stained green whereas cytoplasm was counterstained red. Scale bar: 100 μm

    Journal: World Journal of Microbiology & Biotechnology

    Article Title: Chlamydia trachomatis disrupts host metabolism in primary cervical epithelial cells

    doi: 10.1007/s11274-025-04584-4

    Figure Lengend Snippet: C. trachomatis infection of primary cervical epithelial cells. A Growth curve for C. trachomatis infection in primary cervical epithelial cells over a time course of 96 h. The chlamydial genome copy number was normalized to that of the host and the results were expressed as mean ± standard error (SE) of two independent experiments performed in triplicates. Significance is indicated as * P < 0.05 and ** P < 0.01. B Representative images of C. trachomatis -infected primary cervical epithelial cells at 24 h.p.i., and 48 h.p.i. EBs (dashed arrows) and RBs (solid arrows) were stained green whereas cytoplasm was counterstained red. Scale bar: 100 μm

    Article Snippet: Cervical epithelial cells (PCS-480-011) (ATCC, VA, USA) were cultured in cervical epithelial cell basal medium supplemented with cervical epithelial cell growth kit (ATCC PCS-480-042).

    Techniques: Infection, Staining

    Temporal gene expression changes of primary cervical epithelial cells upon C. trachomatis infection. A Volcano plot of DEG between C. trachomatis infection vs. uninfected control. Transcriptional changes in primary cervical epithelial cells with C. trachomatis infection 8, 24, and 40 h vs. uninfected control. Up (log 2 FC ≥ 1) and downregulated (log 2 FC ≤ − 1). genes are represented in red and blue, respectively with P adj < 0.05. B Bar plot showing GSEA of MSigDB hallmark signatures during C. trachomatis infection. Bars in red and blue respectively represent up and downregulated pathways within the MSigDB Hallmark gene set collection based on their normalized enrichment score (NES). Only significantly modified pathways with P adj < 0.05 are shown. C GSEA enrichment plots indicate C. trachomatis -associated downregulation of OXPHOS genes

    Journal: World Journal of Microbiology & Biotechnology

    Article Title: Chlamydia trachomatis disrupts host metabolism in primary cervical epithelial cells

    doi: 10.1007/s11274-025-04584-4

    Figure Lengend Snippet: Temporal gene expression changes of primary cervical epithelial cells upon C. trachomatis infection. A Volcano plot of DEG between C. trachomatis infection vs. uninfected control. Transcriptional changes in primary cervical epithelial cells with C. trachomatis infection 8, 24, and 40 h vs. uninfected control. Up (log 2 FC ≥ 1) and downregulated (log 2 FC ≤ − 1). genes are represented in red and blue, respectively with P adj < 0.05. B Bar plot showing GSEA of MSigDB hallmark signatures during C. trachomatis infection. Bars in red and blue respectively represent up and downregulated pathways within the MSigDB Hallmark gene set collection based on their normalized enrichment score (NES). Only significantly modified pathways with P adj < 0.05 are shown. C GSEA enrichment plots indicate C. trachomatis -associated downregulation of OXPHOS genes

    Article Snippet: Cervical epithelial cells (PCS-480-011) (ATCC, VA, USA) were cultured in cervical epithelial cell basal medium supplemented with cervical epithelial cell growth kit (ATCC PCS-480-042).

    Techniques: Gene Expression, Infection, Control, Modification

    Mitochondrial DEGs at different time points post-infection and associated pathway enrichments. A Bar plot showing the number of mitochondrial DEGs at different time points relative to uninfected controls. B Venn diagram of mitochondrial DEGs in primary cervical epithelial cells at indicated time points. DESeq2 analysis identified significant DEGs with P adj < 0.05 and log 2 FC ≥ 1 or ≤ − 1. C Dot plot of the top enriched DEG pathways (GO: BP, Reactome, WikiPathways). Dot size represents gene count per term, and color indicates -log 10 adjusted P -value (< 0.05). D Pie chart summarizing mitochondrial DEG enrichment (GO: BP, Reactome, WikiPathways) using ClueGO/CluePedia in Cytoscape. Each segment reflects the % of genes per group, with significance indicated at * P adj < 0.05 and ** P adj < 0.01

    Journal: World Journal of Microbiology & Biotechnology

    Article Title: Chlamydia trachomatis disrupts host metabolism in primary cervical epithelial cells

    doi: 10.1007/s11274-025-04584-4

    Figure Lengend Snippet: Mitochondrial DEGs at different time points post-infection and associated pathway enrichments. A Bar plot showing the number of mitochondrial DEGs at different time points relative to uninfected controls. B Venn diagram of mitochondrial DEGs in primary cervical epithelial cells at indicated time points. DESeq2 analysis identified significant DEGs with P adj < 0.05 and log 2 FC ≥ 1 or ≤ − 1. C Dot plot of the top enriched DEG pathways (GO: BP, Reactome, WikiPathways). Dot size represents gene count per term, and color indicates -log 10 adjusted P -value (< 0.05). D Pie chart summarizing mitochondrial DEG enrichment (GO: BP, Reactome, WikiPathways) using ClueGO/CluePedia in Cytoscape. Each segment reflects the % of genes per group, with significance indicated at * P adj < 0.05 and ** P adj < 0.01

    Article Snippet: Cervical epithelial cells (PCS-480-011) (ATCC, VA, USA) were cultured in cervical epithelial cell basal medium supplemented with cervical epithelial cell growth kit (ATCC PCS-480-042).

    Techniques: Infection

    Transcriptomic changes of mitochondrial metabolic-related genes during C. trachomatis infection in primary cervical epithelial cells. Heatmap of mitochondrial shows fold-change expression levels of mitoribosome subunits, OXPHOS complexes I-V, their assembly factors, and enzymes of the TCA cycle and fatty acid beta-oxidation. Red: upregulated, blue: downregulated, white: not detected/quantified ( P adj < 0.05 in ≥ 1 group)

    Journal: World Journal of Microbiology & Biotechnology

    Article Title: Chlamydia trachomatis disrupts host metabolism in primary cervical epithelial cells

    doi: 10.1007/s11274-025-04584-4

    Figure Lengend Snippet: Transcriptomic changes of mitochondrial metabolic-related genes during C. trachomatis infection in primary cervical epithelial cells. Heatmap of mitochondrial shows fold-change expression levels of mitoribosome subunits, OXPHOS complexes I-V, their assembly factors, and enzymes of the TCA cycle and fatty acid beta-oxidation. Red: upregulated, blue: downregulated, white: not detected/quantified ( P adj < 0.05 in ≥ 1 group)

    Article Snippet: Cervical epithelial cells (PCS-480-011) (ATCC, VA, USA) were cultured in cervical epithelial cell basal medium supplemented with cervical epithelial cell growth kit (ATCC PCS-480-042).

    Techniques: Infection, Expressing

    Proposed model of cellular metabolism during C. trachomatis infection. Schematic illustration of metabolic substrate/intermediate utilization in primary cervical epithelial cells during C. trachomatis infection, analyzed via the MitoPlate assay. Enzymes: CS (citrate synthase), ACO2 (aconitase 2), IDH (isocitrate dehydrogenase), KDH (α-ketoglutarate dehydrogenase), SCS (succinyl coA synthetase), SDH (succinate dehydrogenase), FH (fumarate hydratase), MDH (malate dehydrogenase). Blue/red indicate increased/decreased substrate utilization. Dashed lines with bars depict hypothetical scenarios

    Journal: World Journal of Microbiology & Biotechnology

    Article Title: Chlamydia trachomatis disrupts host metabolism in primary cervical epithelial cells

    doi: 10.1007/s11274-025-04584-4

    Figure Lengend Snippet: Proposed model of cellular metabolism during C. trachomatis infection. Schematic illustration of metabolic substrate/intermediate utilization in primary cervical epithelial cells during C. trachomatis infection, analyzed via the MitoPlate assay. Enzymes: CS (citrate synthase), ACO2 (aconitase 2), IDH (isocitrate dehydrogenase), KDH (α-ketoglutarate dehydrogenase), SCS (succinyl coA synthetase), SDH (succinate dehydrogenase), FH (fumarate hydratase), MDH (malate dehydrogenase). Blue/red indicate increased/decreased substrate utilization. Dashed lines with bars depict hypothetical scenarios

    Article Snippet: Cervical epithelial cells (PCS-480-011) (ATCC, VA, USA) were cultured in cervical epithelial cell basal medium supplemented with cervical epithelial cell growth kit (ATCC PCS-480-042).

    Techniques: Infection